[1]戴 伟,吴海兰,赵 明,等.COPD大鼠肠粘膜屏障功能障碍的观察研究[J].肠外与肠内营养杂志,2022,(01):18-23.[doi:10.16151/j.1007-810x.2022.01.004]
　DAI Wei,WU Hai-Lan,ZHAO Ming,et al.Observational study of intestinal mucosal barrier dysfunction in chronic obstructive pulmonary disease rats[J].PARENTERAL & ENTERAL NUTRITION,2022,(01):18-23.[doi:10.16151/j.1007-810x.2022.01.004]
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COPD大鼠肠粘膜屏障功能障碍的观察研究
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《肠外与肠内营养》杂志[ISSN:1007-810X/CN:32-1477/R]

卷:
期数:
2022年01期
页码:
18-23
栏目:
论著
出版日期:
2022-01-10

文章信息/Info

Title:
Observational study of intestinal mucosal barrier dysfunction in chronic obstructive pulmonary disease rats
作者:
戴 伟; 吴海兰; 赵 明; 辛晓峰
南京大学医学院附属金陵医院/东部战区总医院干部呼吸内科，江苏南京 210002
Author(s):
DAI Wei;  WU Hai-Lan;  ZHAO Ming;  XIN Xiao-Feng
Department of Respiratory Medicine, General Hospital of Eastern Theater Command/Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu, China
关键词:
慢性阻塞性肺疾病 ; 肠粘膜屏障 ; 紧密连接 ; 细胞因子
Keywords:
Chronic obstructive pulmonary disease;  Intestinal mucosal barrier;  Tight junction;  Cytokines
分类号:
R459.3
DOI:
10.16151/j.1007-810x.2022.01.004
文献标志码:
A
摘要:
目的：观察COPD大鼠肠粘膜屏障结构和功能的改变并探讨其可能机制。 方法：将40只雄性SD大鼠随机分为对照组及COPD组，每组20只。采用单纯烟熏法建立COPD大鼠模型，HE染色观察肺组织、肠组织的病理变化。用蛋白印迹法测定肠组织紧密连接蛋白的表达，分光光度法测定血清二胺氧化酶的活性，高效液相色谱分析法测定尿L/M值，酶联免疫吸附法测定肠组织中细胞因子的表达。 结果：肠组织病理显示COPD组大鼠肠道粘膜可见中性粒细胞浸润，部分区域有粘膜上皮脱落。COPD组肠组织紧密连接蛋白明显降低（P < 0.05）。血清二胺氧化酶水平，尿L/M，肠组织中炎症因子明显增高（P < 0.05）。 结论：COPD大鼠存在肠粘膜屏障结构改变和功能障碍，其机制可能与肠道炎症反应增强有关。
Abstract:
Objective: To observe the changes of intestinal mucosal barrier structure and function in chronic obstructive pulmonary disease (COPD) rats and explore its possible mechanism. Methods: 40 male SD rats were randomly divided into control group and COPD group (n = 20 each). The COPD rat model was established by simple smoking method, and the pathological changes of lung and intestinal tissue were observed by HE staining. The expression of tight junction protein in intestinal tissue was determined by Western blotting, the activity of serum diamine oxidase was determined by spectrophotometry, the L/M ratio in urine was determined by high performance liquid chromatography, and the level of cytokines in intestinal tissue was determined by enzyme-linked immunosorbent assay. Results: Neutrophil infiltration and exfoliation of mucosal epithelium were observed in COPD group, The intestinal tight junction protein in COPD group was significantly lower than that in the control group (P < 0.05). Urine L/M serum diamine oxidase levels, the inflammatory cytokines in intestinal tissue were significantly higher than those in the control group (P < 0.05). Conclusion: There are structural changes and dysfunction of intestinal mucosal barrier in COPD rats, which may be related to the enhanced intestinal inflammatory response.

参考文献/References:


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备注/Memo

备注/Memo:
作者简介 ：戴 伟，医学硕士研究生，从事呼吸内科疾病研究。E-mail: 271373473@qq.com 通讯作者 ：辛晓峰，Email：xxf__1106@sina.com
更新日期/Last Update: 1900-01-01