[1]谈善军,孟庆洋,庄秋林,等.美托洛尔对大鼠腹腔暴露后肠黏膜屏障的影响[J].肠外与肠内营养杂志,2020,(05):261-264.[doi:10.16151/j.1007-810x.2020.04.002]
 TAN Shan-jun,MENG Qing-yang,ZHUANG Qiu-lin,et al.Effect of metoprolol on intestinal mucosal barrier in rats after peritoneal air exposure[J].PARENTERAL & ENTERAL NUTRITION,2020,(05):261-264.[doi:10.16151/j.1007-810x.2020.04.002]
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美托洛尔对大鼠腹腔暴露后肠黏膜屏障的影响
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《肠外与肠内营养》杂志[ISSN:1007-810X/CN:32-1477/R]

卷:
期数:
2020年05期
页码:
261-264
栏目:
论著
出版日期:
2020-09-10

文章信息/Info

Title:
Effect of metoprolol on intestinal mucosal barrier in rats after peritoneal air exposure
作者:
谈善军孟庆洋庄秋林奚秋磊蒋 奕吴国豪
复旦大学附属中山医院普通外科 上海市临床营养研究中心,上海 200032
Author(s):
TAN Shan-jun MENG Qing-yang ZHUANG Qiu-lin XI Qiu-lei JIANG Yi WU Guo-hao
Department of General Surgery/Shanghai Clinical Nutrition Research Center, Zhongshan Hospital,Fudan University, Shanghai 200032, China
关键词:
美托洛尔 肠黏膜屏障 细菌易位 腹部手术 腹腔暴露
Keywords:
Metoprolol Intestinal mucosal barrier Bacterial translocation Abdominal surgery Open abdomen
分类号:
R574
DOI:
10.16151/j.1007-810x.2020.04.002
文献标志码:
A
摘要:
目的:探讨美托洛尔对大鼠腹腔暴露后肠黏膜屏障的保护作用。 方法:建立腹腔暴露大鼠模型,将 24只大鼠随机分为三组:对照组、腹腔暴露组和美托洛尔组(腹腔暴露+美托洛尔干预,5 mg/kg),每组8只。观察各组大鼠小肠组织的病理变化,检测体外肠道通透性和血清D-乳酸变化,并通过培养肝、脾、肠系膜淋巴结细菌观察肠道细菌易位情况。 结果:腹腔暴露组较对照组大鼠肠黏膜损伤Chiu’s评分,体外肠道通透性,血清D-乳酸和肠道细菌易位明显增加(P <0.05);美托洛尔组较腹腔暴露组大鼠肠黏膜损伤Chiu’s评分,体外肠道通透性,血清D-乳酸和肠道细菌易位明显降低(P < 0.05)。 结论:美托洛尔能降低腹腔暴露后的肠黏膜损伤,有助于保护腹部手术后的肠黏膜屏障功能。
Abstract:
Objective: To investigate the protective effect of metoprolol on intestinal mucosal barrier after open abdomen in rats. Methods: A rat model of open abdomen was established. Twenty-four rats were randomized into three groups: control group, open abdomen group, and metoprolol group (open abdomen plus 5 mg/kg metoprolol intervention), respectively (n=8 each). The terminal ileum was sampled to observe histological changes and to analyze Chiu’s score for damage of intestinal mucosa. Blood was obtained to determinate serum D-lactate; terminal ileum was sampled to determinate in vitro intestinal permeability; liver, spleen and mesenteric lymph nodes were excised for evaluation of bacterial translocation. Results: Compared to control group, open abdomen group showed a significant increase in Chiu’s score for damage of intestinal mucosa, serum D-lactate, in vitro intestinal permeability, and bacterial translocation rate (P < 0.05). Compared to open abdomen group, metoprolol group showed a significant decrease in Chiu’s score for damage of intestinal mucosa, serum D-lactate, in vitro intestinal permeability, and bacterial translocation rate (P < 0.05). Conclusion: The data suggest that metoprolol could decrease intestinal mucosal damage induced by open abdomen, which is conducive to maintain intestinal mucosal barrier function after abdominal surgery.

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备注/Memo

备注/Memo:
基金项目 :国家自然科学基金青年科学基金项目(81900484);中国博士后科学基金资助项目(2019M661370);上海市青年科技英才扬帆计划(18YF1404700);上海市自然科学基金(19ZR1409100);上海市卫生健康系统重要薄弱学科建设计划-临床营养学(2019ZB0105);复旦大学附属中山医院青年科学基金(2016ZSQN56) 作者简介 :谈善军,医学博士后,从事普通外科和临床营养专业。E-mail: shanjuntan@163.com 通讯作者 :吴国豪,E-mail: prowugh@163.com
更新日期/Last Update: 1900-01-01